Productive Herpes Simplex Type 1 infection alters mitochondrial biogenesis in human epithelial cells
Productive Herpes Simplex Type 1 infection alters mitochondrial biogenesis in human epithelial cells
DOI:
https://doi.org/10.56754/0718-9958.2024.218Keywords:
HSV-1, Keratinocytes, Organelle BiogenesisAbstract
- Introduction: HSV-1 infection has been studied in neurons, where an alteration of cell signaling pathways has been observed during infection, using energy, apoptotic and cell survival pathways.
- Objective: To characterize the effects of productive HSV-1 infection on the mitochondrial biogenesis of HaCaT cells derived from human keratinocytes.
- Materials and Methods: Various techniques will be performed such as cell culture, indirect immunofluorescence and viral infection.
- Results: HSV-1 infection generated a redistribution of mitochondria in the perinuclear zone, given the immunoreaction of ICP8 and TOM40, which showed that there was positive reactivity, and that it has an effect on mitochondrial density.
- Discussion: It is important to study the perinuclear pattern and the colocalization with other structures of the TOM40 protein compared to the control.
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Copyright (c) 2024 Josefa Elmohrez Rosas, Benjamín Oporto Barría
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