Efectos del Ozono en la Regeneración Nerviosa en Ratas. Una Revisión Sistemática.

Autores/as

  • Abdías Cofré Morales Universidad de la Frontera
  • Gabriela Cofré Morales
  • Elías Figueroa Villalobos

Palabras clave:

ozono, metilprednisolona, regeneración nerviosa, agentes neuroprotectores

Resumen

ANTECEDENTES: Agentes antioxidantes como el ozono se han utilizado previamente para tratar lesiones nerviosas. Este, en particular, presenta potencial como terapia neuroregenerativa no invasiva debido a su mecanismo de acción. Sin embargo, existen pocos estudios experimentales sobre ozonoterapia y su efecto sobre regeneración nerviosa periférica.

OBJETIVO: Evaluar los efectos de la ozonoterapia (TO) sobre lesiones nerviosas en ratas.

MÉTODO: Para esta revisión sistemática, se consideraron estudios en inglés publicados entre los años 2015 y el 15 de mayo del 2020 con las palabras clave Ozone AND "nerve regeneration”, obtenidos de bases de datos como: Medline (Pubmed), SCOPUS, Web of Science, EMBASE, Clinical Key y Journal of Neurosurgery. La calidad metodológica se evaluó mediante la pauta ARRIVE.

RESULTADOS: De un total de 38 resultados, se incluyeron 7 artículos de acuerdo a los criterios de inclusión. Estos comprenden una cantidad de sujetos de prueba entre 14 y 100 ratas, en donde evaluaron los efectos del ozono en la regeneración nerviosa dentro de un periodo de 7 y 90 días. Los siete estudios arrojaron resultados positivos sobre el tratamiento de lesiones nerviosas con ozono, dos estudios indicaron resultados superiores al utilizar ozono y metilprednisolona combinados.

DISCUSIÓN: Si bien la evidencia recabada fue limitada, la TO demostró capacidades neuroprotectoras y promotoras de regeneración nerviosa al contribuir con la creación de un microambiente favorable para este proceso, capeando los efectos del estrés oxidativo sobre el tejido. La TO complementada con metilprednisolona fue superior a la TO por sí sola, observándose una mayor respuesta antiinflamatoria y antioxidante, con menor índice de degeneración. Esto sugiere un posible potenciamiento de las cualidades neuroprotectoras de ambos tratamientos. Estos hallazgos indican la posibilidad de desarrollar terapias de regeneración nerviosa basadas en ozono en el futuro.

 

 

Citas

REFERENCIAS

Faroni A, Mobasseri A, Kingham P, Reid A. Peripheral nerve regeneration: Experimental strategies and future perspectives. Adv. Drug Deliv. Rev [Internet] 2015 [consultado 2020 Jun 19]; 82-83:160–167. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0169409X14002737?via%3Dihub DOI https://doi.org/10.1016/j.addr.2014.11.010

Sulaiman W, Gordon T. Neurobiology of Peripheral Nerve Injury, Regeneration, and Functional Recovery: From Bench Top Research to Bedside Application. Ochsner Journal [Internet] 2013 [consultado 2020 Ago 13]; 13(1):100-108. Disponible en: http://www.ochsnerjournal.org/content/13/1/100?utm_source=TrendMD&utm_medium=cpc&utm_campaign=Ochsner_Journal_TrendMD_1

Kim T, Yoon S., Lee W, Kim J, Shin J, Lee S, Lee S. Protective effect of GCSB-5, an herbal preparation, against peripheral nerve injury in rats. J. Ethnopharmacol [Internet] 2011 [consultado 2020 Ago 14]; 136(2):297-304. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0378874111002777?via%3Dihub DOI 10.1016/j.jep.2011.04.037

Hervera A, De Virgiliis F, Palmisano I, Zhou L, Tantardini E, Kong G, et al. Reactive oxygen species regulate axonal regeneration through the release of exosomal NADPH oxidase 2 complexes into injured axons. Nat Cell Biol [Internet] 2018 [consultado 2020 Ago 14]; 20(3):307-319. Disponible en: https://www.nature.com/articles/s41556-018-0039-x DOI 10.1038/s41556-018-0039-x

Ozbay I, Ital I, Kucur C, Akcılar R, Deger A, Aktas S, et al. Effects of ozone therapy on facial nerve regeneration. BJORL [Internet] 2017 [consultado 2020 Jun 15]; 83(2):168-175. Disponible en: https://www.sciencedirect.com/science/article/pii/S1808869416300453?via%3Dihub DOI https://doi.org/10.1016/j.bjorl.2016.02.009

Smith N, Wilson A, Gandhi J, Vatsia S, Khan S. Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res [Internet] 2017 [consultado 2020 Ago 16]; 7(3):212-219. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674660/ DOI https://doi.org/10.4103/2045-9912.215752

Bocci V, Borrelli E, Travagli V, Zanardi I. The ozone paradox: ozone is a strong oxidant as well as a medical drug. Med. Res. Rev. [Internet] 2009 [consultado 2020 Ago 20]; 29(4):646-682. Disponible en: https://pubmed.ncbi.nlm.nih.gov/19260079/ DOI 10.1002/med.20150

Saini R. Ozone therapy in dentistry: A strategic review. J Nat Sci Biol Med [Internet] 2011 [consultado 2020 Jun 20]; 2(2):151-153. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276005/ DOI 10.4103/0976-9668.92318

Elvis A, Ekta J. Ozone therapy: A clinical review. J Nat Sci Biol Med [Internet] 2011 [consultado 2020 Ago 16]; 2(1):66-70. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312702/ DOI 10.4103/0976-9668.82319

Yucesoy T, Kutuk N, Canpolat D, Alkan A. Comparison of Ozone and Photo-Biomodulation Therapies on Mental Nerve Injury in Rats. J Oral Maxillofac Surg [Internet] 2017 [consultado 2020 Jun 15]; 75(11):2323-2332. Disponible en: https://linkinghub.elsevier.com/retrieve/pii/S0278-2391(17)30472-X DOI 10.1016/j.joms.2017.04.016

Menorca R, Fussel T, Elfar J. Peripheral Nerve Trauma: Mechanisms of Injury and Recovery. Hand Clin [Internet] 2013 [consultado 2021 Mar 9]; 29(3):317-330. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4408553/ DOI 10.1016/j.hcl.2013.04.002

Somay H, Emot ST, Uslu S, Orakdogen M, Meric ZC, Ince U, et al. The histological effects of ozone therapy on sciatic nerve crush injury in rats. World Neurosurg [Internet] 2017 [consultado 2021 Mar 9]; 105:702-708. Disponible en: https://pubmed.ncbi.nlm.nih.gov/28587982/ DOI 10.1016/j.wneu.2017.05.161

Kalender A, Dogan A, Bakan V, Yildiz H, Gokalp M, Kalender M. Effect of Zofenopril on regeneration of sciatic nerve crush injury in a rat model. J Brachial Plex Peripher Nerve Inj [Internet] 2009 [consultado 2021 Mar 9]; 4,6. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700796/ DOI 10.1186/1749-7221-4-6

Gordon T, Sulaiman O, Boyd JG. Experimental strategies to promote functional recovery after peripheral nerve injuries. J Peripher Nerv Syst [Internet] 2003 [consultado 2021 Mar 9]; 8(4):236-50. Disponible en: https://pubmed.ncbi.nlm.nih.gov/14641648/ DOI 10.1111/j.1085-9489.2003.03029.x

Qian Y, Han Q, Zhao X, Song J, Cheng Y, Fang Z, et al. 3D melatonin nerve scaffold reduces oxidative stress and inflammation and increases autophagy in peripheral nerve regeneration. J Pineal Res [Internet] 2018 [consultado 2021 Mar 9]; 65(4):e12516. Disponible en: https://pubmed.ncbi.nlm.nih.gov/29935084/ DOI 10.1111/jpi.12516

Kahraman A, Kahveci R. Evaluating the effect of polytetrafluoroethylene and extractum cepae-heparin-allantoin gel in peripheral nerve injuries in a rat model. Plast Surg (Oakv) [Internet] 2015 [consultado 2021 Mar 9]; 23(1):9-14. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364148/ DOI https://doi.org/10.4172/plastic-surgery.1000902

Ogut E, Yildirim F, Sarikcioglu L, Aydin M, Demir N. Neuroprotective Effects of Ozone Therapy After Sciatic Nerve Cut Injury. Kurume Med J [Internet] 2020 [consultado 2020 Jun 16]; 65(4):137-144. Disponible en: https://www.jstage.jst.go.jp/article/kurumemedj/65/4/65_MS654002/_article DOI https://doi.org/10.2739/kurumemedj.MS654002

Clavo B, Rodríguez-Esparragón F, Rodríguez-Abreu D, Martínez-Sánchez G, Llontop P, Aguiar-Bujanda D, et al. Modulation of Oxidative Stress by Ozone Therapy in the Prevention and Treatment of Chemotherapy-Induced Toxicity: Review and Prospects. Antioxidants (Basel) [Internet] 2019 [consultado 2021 Mar 9] 8(12):588. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943601/ DOI 10.3390/antiox8120588

Yüce S, Cemal Gökçe E, Işkdemir A, Koç E, Cemil D, Gökçe A, et al. An experimental comparison of the effects of propolis, curcumin, and methylprednisolone on crush injuries of the sciatic nerve. Ann Plast Surg [Internet] 2015 [consultado 2020 Jun 25]; 74(6):684-692. Disponible en: https://journals.lww.com/annalsplasticsurgery/Abstract/2015/06000/An_Experimental_Comparison_of_the_Effects_of.11.aspx DOI 10.1097/SAP.0000000000000026

Yılmaz Z, Senoglu M, Kurutas E, Ciralik H, Ozbag D. Neuroprotective effects of mannitol and vitamin C on crush injury of sciatic nerve; an experimental rat study. J Neurol Sci [Internet] 2012 [consultado 2020 Jul 2]; 28:538-551. Disponible en: https://www.researchgate.net/publication/287015552_Neuroprotective_Effects_of_Mannitol_And_Vitamin_C_on_Crush_Injury_of_Sciatic_Nerve_An_Experimental_Rat_Study

Cherian D, Pete T, Narayanan A, Madhavan, S, Achammada S, Vynat G. Malondialdehyde as a Marker of Oxidative Stress in Periodontitis Patients. J Pharm Bioallied Sci [Internet] 2019 [consultado 2021 Mar 10]; 11(2):297-300. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555357/ DOI 10.4103/JPBS.JPBS_17_19

Gonçalves N, Mohseni S, El Soury M, Ulrichsen M, Richner M, Xiao J, et al. Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression. Front. Cell. Neurosci [Internet] 2019 [consultado 2021 Mar 10]; 13:235. Disponible en: https://www.frontiersin.org/articles/10.3389/fncel.2019.00235/full DOI https://doi.org/10.3389/fncel.2019.00235

Ocejo A, Correa R. Methylprednisolone. StatPearls [Internet] 2020 [consultado 2020 Ago 23]. Disponible en: https://www.ncbi.nlm.nih.gov/books/NBK544340/

Ozbek Z, Aydin HE, Kocman AE, Ozkara E, Sahin E, Bektur E, et al. Neuroprotective Effect of Genistein in Peripheral Nerve Injury. Turk Neurosurg [Internet] 2017 [consultado 2021 Mar 10]; 27(5):816-822. Disponible en: https://pubmed.ncbi.nlm.nih.gov/27759874/ DOI 10.5137/1019-5149.JTN.18549-16.1

Ozturk O, Tezcan A, Adali Y, Yıldırım C, Aksoy O, Yagmurdur H, et al. Effect of ozone and methylprednisolone treatment following crush type sciatic nerve injury. Acta Cir. Bras [Internet] 2016 [consultado 2020 Jun 15]; 31(11):730-735. Disponible en: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016001100730&lng=en&tlng=en DOI https://doi.org/10.1590/s0102-865020160110000005

Meier R, Boddington S, Krug C, Acosta F, Thullier D, Henning T, et al. Detection of postoperative granulation tissue with an ICG-enhanced integrated OI-/X-ray System. J Transl Med [Internet] 2008 [consultado 2021 Mar 10]; 6:73. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613387/#B3 DOI 10.1186/1479-5876-6-73

Gürkan G, Sayin M, Kizmazoglu C, Erdogan M, Yigitturk G, Erbak Y, et al. Evaluation of the neuroprotective effects of ozone in an experimental spine injury model. J Neurosurg: Spine [Internet] 2020 [consultado 2020 Jun 15]; 1-9. Disponible en: https://thejns.org/spine/view/journals/j-neurosurg-spine/33/3/article-p406.xml DOI https://doi.org/10.3171/2020.2.SPINE191439

Eker E, Cok O, Aribogan A, Arslan G. Management of Neuropathic Pain with Methylprednisolone at the Site of Nerve Injury. Pain Med [Internet] 2012 [consultado 2021 Mar 10]; 3:443-451. Disponible en: https://academic.oup.com/painmedicine/article/13/3/443/1852083 DOI https://doi.org/10.1111/j.1526-4637.2011.01323.x

Aslan R, Kutlu R, Civi S, Tasyurek E. The correlation of the total antioxidant status (TAS), total oxidant status (TOS) and paraoxonase activity (PON1) with smoking. Clin. Biochem [Internet] 2014 [consultado 2021 Mar 10]; 47(6):393-397. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0009912013004530?via%3Dihub DOI https://doi.org/10.1016/j.clinbiochem.2013.10.002

León O, Menéndez S, Merino N, Castillo R, Sam S, Pérez L, et al. zone oxidative preconditioning: a protection against cellular damage by free radicals. Mediators Inflamm [Internet] 1998 [consultado 2020 Jul 2]; 7(4):289–294. Disponible en: https://www.hindawi.com/journals/mi/1998/793438/ DOI https://doi.org/10.1080/09629359890983

Mashhoudi M, Tajziehchi M, Heidari M, Bushehri A, Moayer F, Mansouri N, et al. Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat. J Lasers Med Sci [Internet] 2017 [consultado 2021 Mar 10]; 8(1):32-37. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5642176/ DOI 10.15171/jlms.2017.s7

Jessen K, Brennan A, Morgan L, Mirsky R, Kent A, Hashimoto Y, et al. The schwann cell precursor and its fate: A study of cell death and differentiation during gliogenesis in rat embryonic nerves. Neuron [Internet] 1994 [consultado 2021 Mar 10]; 12(3):509-527. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/0896627394902097 DOI https://doi.org/10.1016/0896-6273(94)90209-7

Yuca Y, Yucesoy T, Tok O, Alkan A. The efficiency of ozone therapy and low-level laser therapy in rat facial nerve injury. J Craniomaxillofac Surg [Internet] 2020 [consultado 2020 Jun 16]; 48(3):308-314. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S1010518220300251 DOI https://doi.org/10.1016/j.jcms.2020.01.017

Sene G, Sousa F, Fazan V, Barbieri C. Effects of laser therapy in peripheral nerve regeneration. Acta Ortop Bras [Internet] 2013 [consultado 2021 Mar 10]; 21(5):266-270. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3874995/ DOI 10.1590/S1413-78522013000500005

Schwartz-Tapia A, Martínez-Sánchez G, Sabah F. Madrid Declaration on Ozone Therapy; ISCO3 (International Scientific Committee of Ozone Therapy) 2nd ed. [Internet] 2015 [consultado 2020 Ago 16]. Disponible en: http://scholar.google.cl/scholar_url?url=https://www.doktermulder.nl/wp-content/uploads/2020/02/Madrid-Declaration.pdf&hl=es&sa=X&ei=YAAbYIqjJ4OBy9YP0reL0AU&scisig=AAGBfm3Q_d55j2aYDbNxScKTuvq7FyLGgg&nossl=1&oi=scholarr

Hassen, E., mahmod, A., Ibrahem, N., El-Shal, A. The Effect of Long Term Administration of Aspartame on the Sciatic nerve of adult male albino rats and the Possible Therapeutic Role of Ozone (Histological and Biochemical Study). EJH [Internet] 2019 [consultado 2020 Jun 20]; 42(1):191-201. Disponible en: https://ejh.journals.ekb.eg/article_36758.html DOI 10.21608/ejh.2018.6082.1035

Soffritti M, Padovani M, Tibaldi E, Falcioni L, Manservisi F, Belpoggi F. The carcinogenic effects of aspartame: The urgent need for regulatory re-evaluation. Am J Ind Med [Internet] 2014 [consultado 2020 Ago 16]; 57(4):383-397. Disponible en: https://onlinelibrary.wiley.com/doi/abs/10.1002/ajim.22296 DOI 10.1002/ajim.22296

Liu B, Xin W, Tan J, Zhu R, Li T, Wang D, et al. Myelin sheath structure and regeneration in peripheral nerve injury repair. Proc Natl Acad Sci U S A [Internet] 2019 [consultado 2021 Mar 11]; 116(44):22347-22352. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825268/ DOI 10.1073/pnas.1910292116

Liu Z, Jin Y, Chen L, Wang Y, Yang X, Cheng J, et al. Specific marker expression and cell state of Schwann cells during culture in vitro. PloS one [Internet] 2015 [consultado 2021 Mar 11]; 10(4):e0123278. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393255/ DOI 10.1371/journal.pone.0123278

Fang Z, Ge X, Chen X, Xu Y, Yuan W, Ouyang Y. Enhancement of sciatic nerve regeneration with dual delivery of vascular endothelial growth factor and nerve growth factor genes. J Nanobiotechnol [Internet] 2020 [consultado 2021 Mar 11]; 18:46. Disponible en: https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-020-00606-5#citeas DOI https://doi.org/10.1186/s12951-020-00606-5

Nishida Y, Yamada Y, Kanemaru H, Ohazama A, Maeda T, Seo K. Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration. Biomed Res [Internet] 2018 [consultado 2021 Mar 11]; 39(6):287-294. Disponible en: https://pubmed.ncbi.nlm.nih.gov/30531158/ DOI 10.2220/biomedres.39.287

Dun XP, Parkinson DB. Role of Netrin-1 Signaling in Nerve Regeneration. Int J Mol Sci [Internet] 2017 [consultado 2021 Mar 11]; 18(3):491. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372507/ DOI 10.3390/ijms18030491

Tomita Y, Horiuchi K, Kano K, Tatsukawa T, Matsuo R, Hayasaka T, et al. Ninjurin 1 mediates peripheral nerve regeneration through Schwann cell maturation of NG2-positive cells. Biochem Biophys Res Commun [Internet] 2019 [consultado 2021 Mar 11]; 519(3):462-468. Disponible en: https://pubmed.ncbi.nlm.nih.gov/31526566/ DOI 10.1016/j.bbrc.2019.09.007

McGregor CE, English AW. The Role of BDNF in Peripheral Nerve Regeneration: Activity-Dependent Treatments and Val66Met. Front Cell Neurosci [Internet] 2019 [consultado 2021 Mar 11]; 12:522. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/30687012/ DOI 10.3389/fncel.2018.00522

Boyd JG, Gordon T. Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor sustain the axonal regeneration of chronically axotomized motoneurons in vivo. Exp Neurol [Internet] 2003 [consultado 2021 Mar 11]; 183(2):610-9. Disponible en: https://pubmed.ncbi.nlm.nih.gov/14552902/ DOI: 10.1016/s0014-4886(03)00183-3

Feng X, Yuan W. Dexamethasone enhanced functional recovery after sciatic nerve crush injury in rats. Biomed Res Int [Internet] 2015 [consultado 2021 Mar 11]; 2015: 627923. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369935/ DOI 10.1155/2015/627923

Descargas

Publicado

2021-05-07

Cómo citar

1.
Cofré Morales A, Cofré Morales G, Figueroa Villalobos E. Efectos del Ozono en la Regeneración Nerviosa en Ratas. Una Revisión Sistemática. . Rev Estud Med Sur [Internet]. 7 de mayo de 2021 [citado 28 de marzo de 2024];9(1). Disponible en: http://rems.ufro.cl/index.php/rems/article/view/120